만성 치주염 환자에서 stromelysins 및 membrane type의 matrix metalloproteinases 발현에 Diabetes Mellitus의 영향
The Influence of Diabetes Mellitus on Expression of Stromelysins and Membrane type Matrix Metalloproteinases in Human Chronic Periodontitis
류상호, 이재목,
소속 상세정보
류상호 ( Ryu Sang-Ho ) - 경북대학교 치의학전문대학원 치주과학교실
이재목 ( Lee Jae-Mok ) - 경북대학교 치의학전문대학원 치주과학교실
KMID : 1035320140410040263
Abstract
The purposes of this study were to observe influence of diabetes mellitus(DM) on the expression of MMP?3 of stromelysin type and MMP?14 of membrane type matrix metalloproteinase in the gingival tissues of patients with type 2 DM and healthy adults with chronic periodontitis. Gingival samples were devided into three groups. Group 1 is clinically healthy gingiva without bleeding, bone resorption or periodontal pockets. Group 2 is inflammed gingiva from patients with chronic periodontitis. Group 3 is inflammed gingiva from patients with chronic periodontitis associated with type 2 DM. Tissue samples were analyzed by Western blotting. The quantification of MMP?3 and MMP?14 were performed using a densitometer. MMP?3 and MMP?14 expressions were similar in group 1 and 2. While expression of MMP-14 increases in proportion to MMP-3 expression, expression of both type of MMP showed increasing tendency in chronic periodontitis associated to type 2 DM and mean amount of it was more increased in group 3 comparing to other group with statistically significant difference.
In conclusion, MMP?3 and MMP?14 expression levels were similar in healthy and inflamed gingival tissue from systemically healthy patients. And tissue with chronic periodontitis associated to type 2 DM showed significantly increased MMP?3 and MMP?14 levels compared to healthy and non?diabetic inflamed gingiva. Also, MMP?3 may be related to MMP?14 activity in chronic periodontitis associated with type 2 DM. Therefore, the expression levels of MMP?3 and MMP?14 will be inflammatory markers of periodontal inflammed tissue with type 2 DM.
키워드
chronic periodontitis; MMP-3; MMP-14; Type 2 Diabetes Mellitus
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